Advancing the creation of genetically engineered models

Mirimus has harnessed the power of CRISPR-Cas9 to create a novel platform for the generation of designer models by introducing specific genetic alterations in mouse models.

Animal models are the gold standard for dissecting disease mechanisms; however, the cost and long lead time to develop them has prevented their routine use in research. The advent of CRISPR/Cas9 genome editing, enables us to genetically engineer and study human diseases in mice.

Mirimus, together with Charles River Laboratories, provides end-to-end service, beginning with model design, molecular engineering expertise, CRISPR/Cas9 genome engineering and RNAi technologies, and full embryology services. Clients can tap into Mirimus experts in creation of RNAi and CRISPR/Cas9 mouse models for pre-clinical research models that will pave the way toward our understanding of disease pathogenesis and bring us better, faster cures.

Applications

Mimic genetic diseases

Model specific mutations

Gene knockout/knockin models

Reporter models

Conditional knockout models

The mastery of modeling human genetic diseases

Successful mouse model generation begins with superior design strategies. Our CRISPR-Cas9 mouse generation methods lead to success by elegant targeting vector design and thorough validation of CRISPR reagents in vitro prior to mouse generation.

We work with Charles River Laboratory to enable seamless, end-to-end model creation, animal maintenance, colony expansion and implementation of experimental protocols.

Let us guide you through this journey, every step of the way.

CRISPR/Cas9 Based Services:

Indels

Point Mutation

Small Fragment Knockin

Large Fragment Knockin

Knockout/Conditional Knockout

Workflow

Strategy design
Vector construction
Direct injection into mouse embryos
Founder screening
Breeding founders to obtain F1

CRISPR/Cas9-mediated DNA cleavage can be triggered by specific guide RNA sequences to induce:
(1) non-homologous end-joining which will produce insertional or
deleterious mutations (indels)
(2) homology directed repair using a donor template for point mutations or specific transgene insertion. Addition of our HDR selection cassette enables positive selection of ES cell clones that have undergone homologous recombination, yielding higher efficiency in selection of properly targeted clones.

Order your CRISPR/Cas9 mouse model

Gene of Interest
Select your CRISPR/Cas9 Mediated Mouse Service