Animal models are the gold standard for dissecting disease mechanisms; however, the cost and long lead time to develop them has prevented their routine use in research. The advent of CRISPR/Cas9 genome editing, enables us to genetically engineer and study human diseases in mice.
Mirimus, together with Charles River Laboratories, provides end-to-end service, beginning with model design, molecular engineering expertise, CRISPR/Cas9 genome engineering and RNAi technologies, and full embryology services. Clients can tap into Mirimus experts in creation of RNAi and CRISPR/Cas9 mouse models for pre-clinical research models that will pave the way toward our understanding of disease pathogenesis and bring us better, faster cures.
Mimic genetic diseases
Model specific mutations
Gene knockout/knockin models
Conditional knockout models
Successful mouse model generation begins with superior design strategies. Our
We work with Charles River Laboratory to enable seamless,
Let us guide you through this journey, every step of the way.
Small Fragment Knockin
Large Fragment Knockin
deleterious mutations (indels)
(2) homology directed repair using a donor template for point mutations or specific transgene insertion. Addition of our HDR selection cassette enables positive selection of ES cell clones that have undergone homologous recombination, yielding higher efficiency in selection of properly targeted clones.