Coming Soon…

The RNA-guided Cas9 nuclease from the bacterial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be employed for genetic manipulation of any genomic locus by using a 20-nt targeting sequence within its guide RNA. This powerful tool can be used to generate targeted mutations, deletions and insertions at any genomic locus of eukaryotic cells. Mirimus is studying how to adapt this system to mouse engineering to be able to dramatically reduce the time to develop GEMMs. However, certain mutations are embryonic lethal and therefore having a system where mutations can be induced at a specific time point is most desirable. Mirimus’ objective in the future is to have a system for inducible CRISPR/Cas9 expression in vivo [Dow et al. (2015)].

For more information please contact info@mirimus.com.